Jun 14, 2010

KAT'S WORK F.EX

B-logg of 14th of June Monday:

Migrationassay set up:
100608                        Mice arrived.
100616                        Depo-Provera treatment of  5 mice
100621                        HSV-2 infection 5 mice
100628                        Day 7 take blood from 5 mice at 8:00 am.
Timepoint planning preliminary.
Ja dates changed! 2 days ahead. So,

100618                        Depo-Provera treatment of  5 mice
100623                        HSV-2 infection 5 mice
100630                        Day 7 take blood from 5 mice at 8:00 am.


Today:

Analysis of FACS continued.
Another lay out has been done named TEST analysis for each mouse FACS;
in this workspace I try different gatings to find the best one.


Administrative work:
Email about my studyplan.
Email about FACS forum.

Miscellaneous.
Planning of wanted, future confocal microscopy work with guided tour.


Order today:

Fisher Scientific AB (Göteborg)
Product nr:      Product:              Price: 4790kr

37503 Pierce Rapid ELISA Mouse mAb Isotyping Kit
Five microplates and sufficient reagents to isotype 60 mouse monoclonal antibodies.
Includes:
Pre-coated 96-well Isotyping Plates, 5 plates
Goat Anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30 ml
TMB Substrate, 55 ml
Stop Solution, 55 ml
30X Wash Buffer, 200 ml
Tris buffered Saline, 2 packs

 


Call Peter Simon and ask if it is a kit that can be used for determining the concentration of the mAb,  message left on answer machine, try again Tuesday! 


Registered also for  webcast:


Meeting status:   Not started
Starting date:   Wednesday 23 June 2010
Starting time:   14:00,GMT Summer Time (London, GMT+01:00)
Duration:   1 hour
Meeting number:   705 891 511
Meeting password:   None
Teleconference:   Conference Code: 314 943 9336
Standard International +44 207 897 0111
Freephone
United States : 1 866 966 1187
Austria : 0800 005 345
Belgium : 0800 40867
Canada : 1 866 663 6623
Denmark : 808 88437
France : 0805 102 182
Italy : 800 969 145
Netherlands : 0800 022 1251
South Africa : 0800 982 906
Sweden : 0200 884 649
United Kingdom : 0800 694 8053
Host's name:   Jeremy Fry 

Host's Email:  jfry@proimmune.com  









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