ANGEL MUSIC

Listen to the most wonderful opera singer,

Romanian of course, Angela Gheorghiu.

ANGELA GHEORGHIU

RIDICULE

One of the favorite (Romanian) authors during my upbringing was Eugene Ionescu.
He was one of the authors of the Theatre d'Absurd (Theatre of the absurd).

The Theatre of Absurd constitutes plays written by playwrights from the 1940s, 1950s and 1960s. The style of theatre was evolved from their work. Their philosophy was that in a godless universe, human existence has no meaning or purpose and therefore all communication breaks down.
Logical construction and argument gives way to irrational and illogical speech and to its ultimate conclusion, silence.

Theme is absurdity.

I hear...

A man's reaction to a world apparently without meaning or man as a puppet controlled or menaced by an invisible outside force.

Someone is keeping me prisoner as we speak...

Seriously, life is comic, mixed with tragical events, hopeless situations, repetitive actions (were they all PhD students actually?), dialogues full of cliches, wordplay and pure NONSENSE. Plots were cyclical or absurdly expansive (Wagner?), either a parody or dismissal of realism and the concept of 'well-made play'.

Ah, I found my way home!

Other playwrights associated with the Theatre of the Absurd are: Samuel Beckett, Arthur Adamov,  Jean Genet, Harold Pinter, Tom Stoppard, Friedrich Durrenmatt, Fernando Arrabal and Edward Albee.

These authors obviously gave voice to Albert Camus' philosophy that life is inherently without meaning.

Mode is set to tragicomedy.

'NOTHING IS FUNNIER THAN UNHAPPINESS... IT'S THE MOST COMICAL THING IN THE WORLD!'  (Ahahahaha, it so is!!)

So, Pataphysics is the science of imaginatory solutions. I wonder if Nature will accept this?

Jean-Paul Sartre was the philosophical spokesman for Existentialism in Paris (expressed in being and Nothingness?).

Ionescu hated Sartre bitterly.

He accused Sartre of supporting Communism but ignoring the atrocities committed by Communists, so Ionesco wrote Rhinoceros as a criticism of blind conformity; at the end of the play, one man remains on Earth resisting transformation into a rhinoceros.

Ionescu wrote The Bald Soprano (La Cantatrice Chauve), The Lesson (La Lecon) and The Chairs (Les Chaises) and Macbett (1972-they year I was born) among others.

The Chairs was on SVT many years ago, but I would like to see it again sometime, preferably in the theatre. How absurd is it that this would probably not happen?

Unless I put up the production myself?

ALL NIGHT LONG

ALEXANDRA IS OUT ALL NIGHT LONG

OH YES!

I managed! I ran out after I saw this view from my bedroom. Happiest moment of the day! =)

Listen to:
The Sky is Broken 

The Moon and The Sky 

VAMPIRE WEEKEND

yukai yottsu shichigatsu

HOMEDMADE YOGHURT BLEV PANNACOTTA

Organic vanilla powder from Coop. Delicious.

Listen to Yoghurt Music:

LUCIEN-N-LUCIANO 'YOGHURT PRESSURE'

SOMMAR

GOD NATT

Göteborg. I am still here.

I jumped up quickly and threw myself at the door. I got so scared of the darkness. I really want to go to a blind cafe. Just to experience what it is like to be in their world.

IT'S EASY

To blog.

Aaooow!! It hurts!
G. D. did you have to infect my spine too HSV-2?


Really. It hurts.

You don't believe me? Take a look.
I told you.

POURQUOI

this life is for herpes, not for me

you managed big time, more than I could ever have imagine

but today I thank the Lord for reminding me what it means to be healthy

and for putting food on my table

Really. I do.

AND THIS IS WHAT IT LOOKS LIKE WHEN U DANCE LIKE ONLY YOU THINK YOU CAN

On my way

Despite my good dancing shoes I almost broke my back, so now I have to rest for like 3 weeks.  I jumped too high, thought I was Niklas for a second...

V AS IN HERPES

Virion polypeptides

HSV-1 virions has been suggested  to contain at least 30 distinct proteins.
They were designated VP and given serial numbers.
All of the virion proteins were made after infection, and no host proteins could be detected in virion preparations.
At least 11 are on the surface of the virion (accessable to antibody) and at least 10 are glycosylated.


GLYCOSYLATION - the enzymatic process that links saccharides to produce glycans, attached to proteins, lipids, or other organic molecules.This enzymatic process produces one of the fundamental biopolymers found in cells (along with DNA, RNA, and proteins). 

Glycosylation is a form of co-translational and post-translational modification. ^1] 
The majority of proteins synthesized in the rough ER undergo glycosylation.
It is an enzyme-directed site-specific process, as opposed to the non-enzymatic chemical reaction of glycation. 
Glycosylation is also present in the cytoplasm and nucleus as the O-GlcNAc modification. 

Five classes of glycans are produced:

• N-linked glycans attached to a nitrogen of asparagine or arginine side chains;
• O-linked glycans attached to the hydroxy oxygen of serine, threonine, tyrosine, hydroxylysine, or hydroxyproline side chains, or to oxygens on lipids such as ceramide;
• phospho-glycans linked through the phosphate of a phospho-serine;
• C-linked glycans, a rare form of glycosylation where a sugar is added to a carbon on a tryptophan side chain;
• glypiation, which is the addition of a GPI anchor that links proteins to lipids through glycan linkages.

The carbohydrate chains attached to the target proteins serve various functions.^[2]
For instance, some proteins do not fold correctly unless they are glycosylated first.^[1]

Also, polysaccharides linked at the amide nitrogen of asparagine in the protein confer stability on some secreted glycoproteins.
Experiments have shown that glycosylation in this case is not a strict requirement for proper folding, but the unglycosylated protein degrades quickly.
Glycosylation may play a role in cell-cell adhesion (a mechanism employed by cells of the immune system), as well.

 

N-linked glycosylation

N-linked glycosylation is important for the folding of some eukaryotic proteins.
The N-linked glycosylation process occurs in eukaryotes and widely in archaea, but very rarely in bacteria.

In Eukaryotes, most N-linked oligosaccharides begin with addition of a 14-sugar precursor to the asparagine in the polypeptide chain of the target protein. 
The structure of this precursor is common to most eukaryotes, and contains 3 glucose, 9 mannose, and 2 N-acetylglucosamine molecules.
A complex set of reactions attaches this branched chain to a carrier molecule called dolichol, and then it is transferred to the appropriate point on the polypeptide chain as it is translocated into the ER lumen.
There are three major classes of N-linked saccharides resulting from this core: high-mannose oligosaccharides, complex oligosaccharides and hybrid oligosaccharides.^[2]

• High-mannose is, in essence, just two N-acetylglucosamines with many mannose residues, often almost as many as are seen in the precursor oligosaccharides before it is attached to the protein.

• Complex oligosaccharides are so named because they can contain almost any number of the other types of saccharides, including more than the original two N-acetylglucosamines.

Proteins can be glycosylated by both types of oligosaccharides on different portions of the protein. Whether an oligosaccharide is high-mannose or complex is thought to depend on its accessibility to saccharide-modifying proteins in the Golgi.
If the saccharide is relatively inaccessible, it will most likely stay in its original high-mannose form.
If it is accessible, then it is likely that many of the mannose residues will be cleaved off and the saccharide will be further modified by the addition of other types of group as discussed above.

The oligosaccharide chain is attached by oligosaccharyltransferase to asparagine occurring in the tripeptide sequence Asn-X-Ser or Asn-X-Thr where X could be any amino acid except Pro.
This sequence is known as a glycosylation sequon.

After attachment, once the protein is correctly folded, the three glucose residues are removed from the chain and the protein is available for export from the ER. 

The glycoprotein thus formed is then transported to the Golgi where removal of further mannose residues may take place.

However, glycosylation itself does not seem to be as necessary for correct transport targeting of the protein, as one might think. Studies involving drugs that block certain steps in glycosylation, or mutant cells deficient in a glycosylation enzyme, still produce otherwise-structurally-normal proteins that are correctly targeted, and this interference does not seem to interfere severely with the viability of the cells.
Mature glycoproteins may contain a variety of oligomannose N-linked oligosaccharides containing between 5 and 9 mannose residues. Further removal of mannose residues leads to a 'core' structure containing 3 mannose, and 2 N-acetylglucosamine residues, which may then be elongated with a variety of different monosaccharides including galactose, N-acetylglucosamine, N-acetylgalactosamine, fucose and sialic acid.
GalNAc, glucose, and rhamnose linked to asparagines have been observed as well, although mostly in less complex organisms or bacteria. Glucose linked to the guanidinium group of arginine in sweet corn amyelogenin is the only reported example of N-linked glycosylation on an amino acid other than asparagine.

O-linked glycosylation

O-N-acetylgalactosamine (O-GalNAc)

O-linked glycosylation occurs at a later stage during protein processing, probably in the Golgi apparatus.

This is the addition of N-acetyl-galactosamine to serine or threonine residues by the enzyme UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (EC 2.4.1.41), followed by other carbohydrates (such as galactose and sialic acid). This process is important for certain types of proteins such as proteoglycans, which involves the addition of glycosaminoglycan chains to an initially unglycosylated "proteoglycan core protein." These additions are usually serine O-linked glycoproteins, which seem to have one of two main functions.

One function involves secretion to form components of the extracellular matrix, adhering one cell to another by interactions between the large sugar complexes of proteoglycans.
The other main function is to act as a component of mucosal secretions, and it is the high concentration of carbohydrates that tends to give mucus its "slimy" feel.
Proteins that circulate in the blood are not normally O-glycosylated, with the exception of IgA1 and IgD (two types of antibody) and C1-inhibitor.

HERPES GUIDE

The glycoproteins are:

gB (VP7 and VP8.5)

gC (VP8)

gD (VP17 and VP18)

gE (VP12.3 and VP12.6)

gG

gH

gI

gK

gL

gM

maybe:

gJ (Us5)

gN (Ul49.5)

Virion envelopes also contain at least two and possibly more nonglycosylated intrinsic membrane proteins

I ADMIT

So yes, I like to take pictures including pictures on myself.
Taking pics on myself and make them (try to make them) look good feels quite rewarding.
Why should I show the smallest pore of my skin magnifed to the 10,000, when there are helpful tools called Retouchera, Contrast, Brusförminskning, Saturation, Sharpness, Glow, Warmify, Adobe Premium Design... etc etc.

Also, if I would have more willing models around to photograph there would be more of them. Anyone wants to be my model? At own risk of course, no guarantees other than honest engagement of course. And I don't publish them without consent. Think about it. And it's for free!

BUT. I also did the good deed today, I think. On my way to the gym, I signed up for 'Läkare utan gränser' on the street. This doctor  (MD) lured me into his corrupted 'We save lives'. Yeah right.

Nope, just kidding. We do? For as little as 50sek /month. That's minimum though. You can donate more but not less. I did not give him my account, to withdraw 50kr/month on autogiro. Autogiro is because they don't want to waste our money on papers. Good thinking. They will contact me.

Do you see what I see?

Yes. Well, I somewhat failed with the picture but maybe next time I will get some more frame or composition or whatever it's called in it.

DIARY ON A WARMEST SUMMERDAY/RAINY TUESDAY

Dragging my feet slowly and in despair on the ground, I didn't think I would make it home after such heavy dancing.
I got my 5th (?) picture Catwalk which made me moderatly happy.
I had enough of catwalks I think. It's not exciting to realize that Kat is walking and walking while discovering Catwalks on the walls of Gothenburg.

I am frustrated now. Like this branch of tree glued onto the wall, ripped out from it roots and hang up like....! Usch.
Thank god I found this lovely flash back motif of children's 'kasta stege' (throw ladder)  in the playing ground.

These days I want to be a child again and remember the joy of playing games.

DANCE

working as a researcher means to think outside the box

keep an open mind

so this is the place where great ideas are born during reload of energy and pause for the mind to process what it has been fed

Today I dance aerobics under the coreography of Niklas, to make room for new synapses to connect

INFECTIOUS AGENT

Presence of neutralizing antibodies to HSV were found in the serum of previously infected adults. Only individuals with neutralizing antibodies developed recurrent lesions. Remarkably, measles and rubella antibodies are associated with protection from subsequent episodes of the disease.

National Herpes Hotline 
(919) 361-8488

National Herpes Resource Center 
herpesnet@ashastd.org

 

One significant advance of our understanding of herpes infection was the detection of antigenic differences between HSV types. Already in 1968, antigenic and biologic differences between HSV-1 and HSV-2 were found by Nahmias and Dowdle. The observation was that HSV-1 was more frequently associated with nongenital infection whereas HSV-2 was associated with genital disease.

Infection of epithelial cells in the mucosal surface gives rise to productive replication, resulting in the production of progeny virions, which can spread to infect additional epithelial cells. Virus enters innervating sensory neurons, and nucleocapsids are transported to the neuronal cell body. The viral DNA is released into the neuronal nucleus and circularizes. Circular viral DNA persists in the neuronal cell nucleus, and the latency-associated transcript is expressed. b | Upon reactivation, viral lytic gene expression is initiated, and newly formed capsids are transported to the axonal termini. Infectious virus is released from the axon and infects epithelial cells, resulting in recurrent infection and virus shedding.
 

Oj, I suddenly was attacked by John Blund. Will ferociously continue tomorrow.

God natt, noapte buna, spakujna nocin and good night herpes wherever you are.