Migrationassay set up:
100608 Mice arrived.
100616 Depo-Provera treatment of 5 mice
100621 HSV-2 infection 5 mice
100628 Day 7 take blood from 5 mice at 8:00 am.
Timepoint planning preliminary.Ja dates changed! 2 days ahead. So,
100618
Depo-Provera treatment of 5 mice
100623 HSV-2 infection 5
mice
100630
Day 7 take blood from 5 mice at 8:00 am.
Today:
Analysis of FACS continued.
Another lay out has been done named TEST analysis for each mouse FACS;
in this workspace I try different gatings to find the best one.
Administrative work:
Email about my studyplan.
Email about FACS forum.
Miscellaneous.
Planning of wanted, future confocal microscopy work with guided tour.
Order today:
Fisher Scientific AB (Göteborg)
Product nr: Product: Price: 4790kr
37503 | Pierce
Rapid ELISA Mouse mAb Isotyping Kit Five microplates and sufficient reagents to isotype 60 mouse monoclonal antibodies. Includes: Pre-coated 96-well Isotyping Plates, 5 plates Goat Anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30 ml TMB Substrate, 55 ml Stop Solution, 55 ml 30X Wash Buffer, 200 ml Tris buffered Saline, 2 packs
Call Peter Simon and ask if it is a kit that can be used for determining the concentration of the mAb, message left on answer machine, try again Tuesday!
Registered also for webcast:
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