REFLECTIONS

KÄRA GULDHEDSGATAN

Hur ska man kunna skilja sig från denna plätt? På väg hem idag tog jag 43 bilder men som vanligt, blir det bara typ två som är OK. Dessa togs med min moble phone (not iPhone) och jag är rätt glad för det.

                   

Rainy evening - thank god number 6 came when I called it.

Tram goes really quickly, I had to hold on to the chair not to fall down on the disgusting floor.

Are there two directions?!

And what do u know, all of a sudden I find myself at good old Järntorget.

Back and forth, back and forth. Why doesn't people protest to this blessed every day life?

Don't you get tired going back and forth and back?

What if herpes would go back and forth, back and forth up down the ganglion highway?

Oh...
Don't you just die from lack of variation, of knowledge, of new people to look at, of new buildings to see, new streets to walk, new air to breath, new latte to hunt for??

I do.

What can you do?

Just take another step in the forward direction.

Once you decide to walk there is no going back.

Sorry.

This picture is extremely annoying, and the reason to why I put it up. It is my leg yes, but that is not important here. I was trying to catch something from a different angle (?) but people were pushing me from behind, screaming at me to stop taking pictures, hitting me on the head with their big Liseberg-won DAJM. Aj!

That's how annoying it is. I didn't manage to make anything from this.

NO POPULATION

This is the last post I make about Flow Jo analysis, don't want to infect the blog with this too...

But what really is up with this program, why, why , why do you eat hours of my time like this!!

I am calling Flow Jo company as we speak! It is enough, I mean that's the least you can do Jo, to save the work I put into your space!

JANE GOODALL

Wonderful news on TV4 today about Jane Goodall and her work with chimpanzees.

She listed two examples of memorable moments during her time with them:

• first one was when one of her chimpanzees who was especially shy to humans, who let her 5 month baby get close to Jane after which her baby touched Jane's nose!

• second when she gave a fruit to one of the chimpanzees (David). She was sitting next to David when she gave it to him, but David showed her that he did not want it. He finally took the fruit and dropped it on the ground, giving Jane a sharp look while squeezing her hand firmly.

This Jane said, was proof of the communication chimpanzees have and which is so similar to humans, dating back to 3-4 million years ago.


In conclusion, Jane has taught us how similar we are to the chimpanzees, even at the point of producing our own tools to use, which we before thought was something unique to humans.

Well, I agree!

These pictures were taken at Mariaplan on my way to work.

CONFIRM KOBE

Dear Ms. Katarina Antonsdotter,

 

We are pleased to announce your presentation time and place as follows. We have assigned a presentation number to your abstract. This number will appear in the congress program, book of abstracts and elsewhere.

 

Presentation Number: not yet public for the blog...

Abstract Title:  not yet public for the blog...

 

 

POSTER PRESENTATION:

Presentation Number of your Poster: not yet public for the blog...

Session Number: not yet public for the blog...

Session Title: Immunity to virus infection (excluding retroviruses)

Presentation Format: Poster

Session Start Date and Time (month/day/year time): 8/24/2010 5:00:00 PM

Session End Date and Time (month/day/year time):: 8/24/2010 6:00:00 PM

 

Location: Kobe International Exhibition Hall

 

For presentation instructions, please visit our website.

http://www.ici2010.org/s_program.html#workshops

 

 GLASS PÅ JOBBET FRÅN ABBOTT:

ER LOGO SER JU NÄSTAN UT SOM GLASS?

KAT'S WORK F.EX

B-logg of 14th of June Monday:

Migrationassay set up:

100608                        Mice arrived.

100616                        Depo-Provera treatment of  5 mice

100621                        HSV-2 infection 5 mice

100628                        Day 7 take blood from 5 mice at 8:00 am.

Timepoint planning preliminary.
Ja dates changed! 2 days ahead. So,

100618                        Depo-Provera treatment of  5 mice

100623                        HSV-2 infection 5 mice

100630                        Day 7 take blood from 5 mice at 8:00 am.

Today:

Analysis of FACS continued.
Another lay out has been done named TEST analysis for each mouse FACS;
in this workspace I try different gatings to find the best one.


Administrative work:
Email about my studyplan.
Email about FACS forum.

Miscellaneous.
Planning of wanted, future confocal microscopy work with guided tour.


Order today:

Fisher Scientific AB (Göteborg)
Product nr:      Product:              Price: 4790kr

37503

Pierce Rapid ELISA Mouse mAb Isotyping Kit Five microplates and sufficient reagents to isotype 60 mouse monoclonal antibodies. Includes: Pre-coated 96-well Isotyping Plates, 5 plates Goat Anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30 ml TMB Substrate, 55 ml Stop Solution, 55 ml 30X Wash Buffer, 200 ml Tris buffered Saline, 2 packs   Call Peter Simon and ask if it is a kit that can be used for determining the concentration of the mAb,  message left on answer machine, try again Tuesday!  Registered also for  webcast: Meeting status:   Not started Starting date:   Wednesday 23 June 2010 Starting time:   14:00,GMT Summer Time (London, GMT+01:00) Duration:   1 hour Meeting number:   705 891 511 Meeting password:   None Teleconference:   Conference Code: 314 943 9336 Standard International +44 207 897 0111 Freephone United States : 1 866 966 1187 Austria : 0800 005 345 Belgium : 0800 40867 Canada : 1 866 663 6623 Denmark : 808 88437 France : 0805 102 182 Italy : 800 969 145 Netherlands : 0800 022 1251 South Africa : 0800 982 906 Sweden : 0200 884 649 United Kingdom : 0800 694 8053 Host's name:   Jeremy Fry  Host's Email:  jfry@proimmune.com

FACS FORUM

Flow Cytometry Forum - Molecular Biology Forum Life Science Forums

Finally, smartest decision of today!

Kind regards,

Ms Flow Jo


Dear Antonsdotter,

Thanks for registering at Molecular Biology Forum Life Science Forums! We are glad you have chosen to be a part of our community and we hope you enjoy your stay.

All the best,
Molecular Biology Forum Life Science Forums

FRY BABY FRY

Halleluja! 

I put the frying pan in dish water and little did I know, that these these organized structures were rearranging meanwhile.

Heat, butter and detergent. That's all you need for this piece of art.

Or is it me that's gone completely crazy, waiting for the frying pan to mature...?

5 year master's degree in music, master's degree in molecular biology and ongoing PhD research,

to find myself blogging about frying pans.

This must come to an end, I promise.

I will train my ability to set the limits.

You see, in my head my allowed bounderies create a huge space around me.

Time to get real.

DRACULA

Sunday dinner in the spirit of my Romanian heritage and Vlad Tepes.

Supa de  Pui (chicken soup)  -  Move over Jamie!

Mititei  cu Salata de iarna  = ('vitlöksjärpar' med vintersallad)

Now I know why Anna Anka likes brosk!

Before frying:

After frying:

I'm so full. I can't move.

SPINNING ELECTRONS

This add on an electron spin was the closest I came to find one which I understood:

Spin-polarized transport 

Spintronics

Electrons have an electrical charge, which enables them to carry an electrical current. However, electrons also have another property, which is the electron spin. Although the electron spin is from quantum mechanical origin and strictly speaking does not have a classical analog, it can be imagined as a "spinning top" which rotates clockwise ("spin-up") or anti-clockwise ("spin-down"). Due to its spin property the electron behaves like a little magnet, which can orient itself along or opposite to an external magnetic field. In the field of spin-based-electronics or "spintronics" one tries to use the electron spin and its sensitivity for a magnetic field in electrical circuits and sensors.
It is our interest is to explore how the electron spin can affect the electron transport properties when it traverses the path from a ferromagnetic material into a non-magnetic material, e.g. a normal metal, a semi-conductor (2DEG) or a superconductor.

(University of Groningen/Physiscs)

FLOW, SWEAT AND MOZZARELLA

You want fame?!? 
Well, fame costs!

N' right here's where you start payin', in sweat!!

=( had to cancel SATS dance aerobic, work out home instead )=

freakin' flow jo

Good food the only thing that gets me through this flow jo Sunday.
I didn't eat on the table, just took away the plate in the photo editing. 

Move over Nigella!

KOBE

How did this end up in my sink? Conference 3017 - I'll be there!

It is time for me to get serious. I have to book hotels in Kobe and flights and start working on my poster. Happy days.


ICI2010   14th International Congress of Immunology, Kobe Japan. August 22-27, during the birthday of Katarina which is onthe 26th of August:

Go to Kobe ICI

Kobe Sannomiya Union
Hotel
(14:00 / 11:00)  

PROF NIKITA'S RESEARCH

Prof Nikita Matsunaga is an Associate Professor in Computational and Physical Chemistry

at the Brooklyn campus of Long Island University.

I had the pleasure of working on one of his projects in the summer of 2001.

My work with him unfortunately ended after I moved back to Sweden after 9/11,

and now looking back at the opportunity I was given, being right in the middle of

adventure, my heart starts beating faster when I read the stuff his doing.

Check this out:

Nikita Matsunagas Webpage 

(proudly find my name on his page Students  -post students... =)

Role of surface crossing

In the undergraduate program, you are taught that a chemical reaction is a 

process that transforms reactants to products via a transition state.

For simple reactions, this is a good picture.

Life is a lot more complicated than man! How does an excited state decay?

How does the spin of an electron get flipped during reaction to get diradicals?

These are the questions we can not answer from the conventional single potential

energy surface picture.

Photochemistry has full of these examples. One of such reactions we are interested in

is called spin-forbidden reaction.

The reaction starts out having all electrons paired (singlet state).

During the reaction, one of the electrons flips its spin to become a diradical state (triplet state).  We need to consider both the singlet and triplet potential energy surfaces.  The most important feature common to both potentials is the lowest energy crossing point between the two states. 

By investigating the minimum energy crossing point, we can learn about what geometry of the molecule the spin is likely to flip as well as probability of the event can be calculated.

 Part of Medicinareberget, Gothenburg.

Unfortunately could not put Nikita's picture of S-T crossing in.

FRUITY

When I was about to pay this the cashier said:

'Do you know that it costs 40kr?'

'Jo jag vet.' =)

'Ok, just wanted to tell you because many people think it is very expensive.'

=)

Now that's what I call service!

ROMANIAN ICRE

When I grew up, this was one of our favorite Romanian entrees on New Year's
dinners or other special events, like birthdays, Easter or parties.

It is a sort of aioli, only with caviar instead of garlic. I used sikrom today, but it
should be löjrom. I just wanted to experiment and see what sik gave me.
Unfortunately disapppointment.

• Put the caviar in a bowl.

• Add a few drops of lemon juice  - this step is very important since lemon makes the                oil go fluffy and white.

• Stir ( I am comfortable with a wooden spoon ) - like you do when you do mayonnaise.

• Add sunflower oil (because it doesn't really have any taste in comparison to olive oil)  little at a time, and be careful not to 'cut' the composition.

•   Keep stirrring while the oil is added, and see how a thick fluffy paste is being                       formed. 

• Continue adding oil, and when it gets too dense (then the volume shrinks) add a few       drops of water. The water will multiply the icre.

• Repeat until you have the desired volume. This varies according to taste. I usually add more caviar because I love caviar. 

• Finish with adding salt after taste, but note that caviar is already salt.

• Serve it on homemade bread =)  or in these little cups from ICA!

LITERATURE

Proudly presents an aquaintance of mine from the days of New York,

on her new novel currently awaiting the Pulitzer Prize Board:

'Sometimes that Happens With Chicken'

Wanda Shapiro

Buy it on Amazon or download it for free at:

Sometimes that Happens With Chicken

Excerpt from the book, chapter 10. It was hard to pick something to post, because everything is so good for showing:

Somehow, Nat pulled it off and no one seems to be the wiser. You’re hearing the story now, but then, in the summer of 1999, no one knew anything about Nat’s building full of Amandas and there aren’t very many things that only one man knows. Even the secret story of Nat’s life has three people completely in the know.
Six Amandas, one more than Nat’s intended five Amandas, live in the very same building and have no idea that they were handpicked from hundreds of rental applications for the single reason that their names are all Amanda.
And why Amandas?
Nat honestly has no idea. Amandas came to him in his sleep whether he wanted them to or not. To his recollection, he has never even met an Amanda. There wasn’t a girl from town when he was in boarding school. His mother had never mentioned any Amandas. His first love wasn’t named Amanda. There wasn’t a pretty girl named Amanda who sat next to him in physics. None of his friends introduced him to their cousin Amanda. There seems to be no reason to explain Nat’s obsession with Amandas other than, of course, his formerly not-so-frequently reoccurring dream.

Also, this quote from Mayowa on www.penswithcojones.com  post on writers quotes I liked:

I am a man, and alive...For this reason I am a novelist. And being a novelist, I consider myself superior to the saint, the scientist, the philosopher, and the poet, who are all great masters of different bits of man alive, but never get the whole hog.
~D.H. Lawrence

The source of today's inspiration!